|
| Status |
Public on Aug 25, 2020 |
| Title |
Cell-type-specific profiling of loaded miRNAs from Caenorhabditis elegans reveals spatial and temporal flexibility in Argonaute loading [miRNA-seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We aimed to characterize the cell-type specific loading patterns of miRNAs in c.elegans. We expressed cell-type specific HA-epitope tagged versions of Argonaute-like 1 (ALG-1) and ALG-2 from three major tissue types i.e. intestine, nervous system and body wall mucle. We found that most miRNAs display highly cell-type specific loading patterns. ALG-1 is more ubiquitously loaded whereas ALG-2 is eniriched for miRNA loading within the nervous system. Addtionally we show that there is flexibility in ALG loading which changes during the aging process.
|
| |
|
| Overall design |
A total of 18 samples were anaysed (9 different stains of c.elegans each with biological replicated including; negative control, ALG-1 and ALG-2 from each of the 3 cell-types intestine, nervous system, body wall muscle and GABAergic neurons). Individual biological samples were immunopreciptiated and purified RNA extracted to assess cell-type specfic loading patterns.
|
| |
|
| Contributor(s) |
Brosnan CA, Palmer AJ, Zuryn S |
| Citation(s) |
33850152 |
| |
| Submission date |
Aug 24, 2020 |
| Last update date |
Apr 15, 2021 |
| Contact name |
Steven Zuryn |
| E-mail(s) |
s.zuryn@uq.edu.au
|
| Organization name |
The University of Queensland
|
| Department |
Queensland Brain Institute
|
| Lab |
Zuryn Lab
|
| Street address |
79 Upland Road
|
| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4067 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL19757 |
Illumina NextSeq 500 (Caenorhabditis elegans) |
|
| Samples (14)
|
|
| This SubSeries is part of SuperSeries: |
| GSE156721 |
Cell-type-specific profiling of loaded miRNAs from Caenorhabditis elegans reveals spatial and temporal flexibility in Argonaute loading |
|
| Relations |
| BioProject |
PRJNA659146 |
| SRA |
SRP278724 |
Less...
More...