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| Status |
Public on Jan 05, 2022 |
| Title |
Single cell RNAseq analysis in embryonic spinal cords to profile transcriptomes in control and Jmjd3cKO motor neurons. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To characterize Jmjd3 function in motor neuron development, we isolated GFP+ cells derived from Olig2 expressing progenitor cells that included motor neuron population and then employed single cell RNAseq. We found some novel motor neuron subtypes in addition to well-known motor columns, and specific genes in each subtype by unbiased and comprehensive analysis. Moreover, by comparing control and Jmjd3cKO motor neuron lineage cells, we found many downregulated and upregulated genes affected by Jmjd3 loss of function. Single cell RNAseq provided very powerful tool to analyze different regulated genes in each subtype rather than overall motor neuron groups.
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| Overall design |
We used Olig2-Cre and mTmG mouse lines to label Olig2-expressing lineage cells which mainly include motor neurons at E12.5 stage and then employed single cell RNAseq of GFP+ cells to profile transcriptomes in control and Jmjd3cKO mice.
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| Contributor(s) |
Wang W, Cho H, Lee S |
| Citation(s) |
35177643 |
| |
| Submission date |
Aug 21, 2020 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Soo-Kyung Lee |
| E-mail(s) |
slee229@buffalo.edu
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| Phone |
716-645-4996
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| Organization name |
University at Buffalo, The State University of New York
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| Department |
Department of Biological Sciences
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| Street address |
109 Cooke Hall
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| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14260 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA658510 |
| SRA |
SRP278363 |
