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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 08, 2020 |
| Title |
Single-cell RNA sequencing of mesenchymal stromal cells from primary donor-matched tissue sources reveals functional heterogeneity in immune activity and cell motility |
| Organism |
Equus caballus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The efficacy of mesenchymal stromal cell (MSC) therapy is thought to depend on the intrinsic heterogeneity of MSC cultures isolated from different tissue sources as well as individual MSCs isolated from the same tissue source, neither of which is well understood. The horse model, in contrast to human and mouse, allows for simultaneous sample collection from multiple tissues of the same animal, which circumvents the inter-donor variation in MSC cultures observed in other models. In the present study, we performed single-cell RNA sequencing (scRNA-seq) on primary equine MSCs that were collected from three donor-matched tissue sources; adipose tissue (AT), bone marrow (BM), and peripheral blood (PB). We observed both inter- and intra-source heterogeneity across the three sources of equine MSCs.
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| Overall design |
Mesenchymal stem cells were derived from 3 donor-matched tissues; adipose tissue, bone marrow, and peripheral blood and subjected to single cell RNA sequencing
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| Contributor(s) |
Harman RM, Patel RS, Fan JC, Park JE, Rosenberg BR, Van de Walle GR |
| Citation(s) |
33276815 |
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| Submission date |
Aug 19, 2020 |
| Last update date |
Dec 09, 2020 |
| Contact name |
Gerlinde Van de Walle |
| E-mail(s) |
grv23@cornell.edu
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| Organization name |
Cornell University
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| Department |
Microbiology and Immunology
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| Lab |
Gerlinde Van de Walle
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| Street address |
235 Hungerford Hill Road
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL21401 |
Illumina NextSeq 500 (Equus caballus) |
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| Samples (3) |
| GSM4732061 |
equineSample_adipose_tissue_derived_MSC_s1 |
| GSM4732062 |
equineSample_bone_marrow_derived_MSC_s2 |
| GSM4732063 |
equineSample_peripheral_blood_derived_MSC_s3 |
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| Relations |
| BioProject |
PRJNA658065 |
| SRA |
SRP278076 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156467_RAW.tar |
52.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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