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Series GSE156467 Query DataSets for GSE156467
Status Public on Dec 08, 2020
Title Single-cell RNA sequencing of mesenchymal stromal cells from primary donor-matched tissue sources reveals functional heterogeneity in immune activity and cell motility
Organism Equus caballus
Experiment type Expression profiling by high throughput sequencing
Summary The efficacy of mesenchymal stromal cell (MSC) therapy is thought to depend on the intrinsic heterogeneity of MSC cultures isolated from different tissue sources as well as individual MSCs isolated from the same tissue source, neither of which is well understood. The horse model, in contrast to human and mouse, allows for simultaneous sample collection from multiple tissues of the same animal, which circumvents the inter-donor variation in MSC cultures observed in other models. In the present study, we performed single-cell RNA sequencing (scRNA-seq) on primary equine MSCs that were collected from three donor-matched tissue sources; adipose tissue (AT), bone marrow (BM), and peripheral blood (PB). We observed both inter- and intra-source heterogeneity across the three sources of equine MSCs.
Overall design Mesenchymal stem cells were derived from 3 donor-matched tissues; adipose tissue, bone marrow, and peripheral blood and subjected to single cell RNA sequencing
Contributor(s) Harman RM, Patel RS, Fan JC, Park JE, Rosenberg BR, Van de Walle GR
Citation(s) 33276815
Submission date Aug 19, 2020
Last update date Dec 09, 2020
Contact name Gerlinde Van de Walle
Organization name Cornell University
Department Microbiology and Immunology
Lab Gerlinde Van de Walle
Street address 235 Hungerford Hill Road
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platforms (1)
GPL21401 Illumina NextSeq 500 (Equus caballus)
Samples (3)
GSM4732061 equineSample_adipose_tissue_derived_MSC_s1
GSM4732062 equineSample_bone_marrow_derived_MSC_s2
GSM4732063 equineSample_peripheral_blood_derived_MSC_s3
BioProject PRJNA658065
SRA SRP278076

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Supplementary file Size Download File type/resource
GSE156467_RAW.tar 52.5 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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