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| Status |
Public on Jan 15, 2021 |
| Title |
The UPR Transducer IRE1 Promotes Breast Cancer Malignancy by Degrading Tumor Suppressor microRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To understand the mechanistic basis by which inositol-requiring enzyme 1 (IRE1) is involved in luminal breast cancer malignancy, we analyzed the transcriptomic signature that IRE1 regulates in breast cancer. We suppressed the activity of IRE1 RNase in luminal breast cancer SUM52 line by adenoviral-based over-expression of the IRE1 dominant-negative K599A or K907A, and then performed RNA-sequencing (RNA-seq) analysis with IRE1 dominant-negative and control SUM52 cells. Through the RNA-seq analysis, we identified 98 genes that were commonly upregulated (65 genes) or downregulated (33 genes) in K599A-expressing SUM52 (SUM52-K599A) or K907A-expressing SUM52 (SUM52-K907A) cells.
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| Overall design |
Total RNAs from SUM52 cells transducted with the IRE1 dominant-negative K599A, K907A, or control adenovirus were harvested, frozen, and sent to the LC Sciences (Houston, TX, USA) for next-generation sequencing.
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| Contributor(s) |
Zhang K, Yang Z |
| Citation(s) |
32911332 |
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| Submission date |
Aug 17, 2020 |
| Last update date |
Jan 15, 2021 |
| Contact name |
Zeng-Quan Yang |
| E-mail(s) |
yangz@karmanos.org
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| Phone |
313-576-8339
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| Organization name |
Karmanos Cancer Institute
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| Department |
Oncology
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| Street address |
4100 John R HWCRC 815
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| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA657549 |
| SRA |
SRP277797 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156332_RAW.tar |
1.8 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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