|
| Status |
Public on Apr 27, 2021 |
| Title |
Non-canonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9 [RIP-seq] |
| Organism |
Campylobacter jejuni subsp. jejuni CG8421 |
| Experiment type |
Other
|
| Summary |
RIP-seq analysis to identify CjCas9 bound RNAs using co-immunoprecipitation and sequencing in CG84-21.
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| |
|
| Overall design |
Cas9 was chromosomally tagged using 3xFLAG epitope at the C-terminus in Campylobacter jejuni CG84-21 wild-type cells and immunoprecipitated using anti-FLAG antibody, untagged version of the wild-type CG84-21 strain was used as a control. Lysates for immunoprecipitation for both untagged and Cas9-3xFLAG samples were prepared from 60 O.D. of bacteria grown to mid-log phase ( OD600 ≈ 0.6) . RNA was eluted from both untagged and Cas9-3xFLAG samples to use for cDNA library preparation and sequencing.
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| |
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| Contributor(s) |
Jiao C, Sharma S, Dugar G, Peeck NL, Bischler T, Wimmer F, Sharma CM, Beisel CL |
| Citation(s) |
33906967 |
| |
| Submission date |
Aug 14, 2020 |
| Last update date |
May 01, 2021 |
| Contact name |
Tom Gräfenhan |
| E-mail(s) |
ht-seq.sysmed@uni-wuerzburg.de
|
| Phone |
+49 - 931 - 31 - 80814
|
| Organization name |
University of Wuerzburg
|
| Department |
Core Unit SysMed
|
| Lab |
Raum D15.02.045
|
| Street address |
Josef-Schneider-Str. 2, Bau D15
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL29023 |
Illumina NextSeq 500 (Campylobacter jejuni subsp. jejuni CG8421) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE156266 |
Non-canonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9 |
|
| Relations |
| BioProject |
PRJNA657241 |
| SRA |
SRP277527 |
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