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| Status |
Public on Oct 06, 2020 |
| Title |
Anti-cancer activity of Cotyledon orbiculata extract |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We investigated the effects of the crude extract of a South African medicinal plant, Cotyledon orbiculata, on cell survival of colon (HCT116) cancer cell lines. Using RNASeq, we discovered that the extract interfered with mRNA regulatory pathways. Here, we found that the extract of Cotyledon orbiculata, a South African medicinal plant, had an anti-proliferative effect in cancer cells, mediated by apoptosis induced by alternative splicing of hnRNPA2B1 and BCL2L1.
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| Overall design |
RNA sequencing was used to determine the effect of crude extract from the Cotyledone orbiculata on differential gene expression and splicing in human colon cancer cells, relative untreated control cells.
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| Contributor(s) |
Bates D, Makhafola TJ, Dlamini Z, Mbele M, Yacqub-Usman K, Hendren A, Haigh D, Blackley Z, Mongan NP, Meyer M |
| Citation(s) |
33163396 |
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| Submission date |
Aug 13, 2020 |
| Last update date |
Nov 16, 2020 |
| Contact name |
Nigel P Mongan |
| Organization name |
University of Nottingham
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| Department |
Biodiscovery Institute
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| Street address |
University Park
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| City |
Nottingham |
| ZIP/Postal code |
NG7 2RD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA657067 |
| SRA |
SRP277442 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156221_A3SS.MATS.ReadsOnTargetAndJunctionCounts.txt.gz |
521.1 Kb |
(ftp)(http) |
TXT |
| GSE156221_A5SS.MATS.ReadsOnTargetAndJunctionCounts.txt.gz |
339.1 Kb |
(ftp)(http) |
TXT |
| GSE156221_Ensembl_Gene_Expression_Counts.txt.gz |
441.3 Kb |
(ftp)(http) |
TXT |
| GSE156221_MXE.MATS.ReadsOnTargetAndJunctionCounts.txt.gz |
249.7 Kb |
(ftp)(http) |
TXT |
| GSE156221_RI.MATS.ReadsOnTargetAndJunctionCounts.txt.gz |
306.2 Kb |
(ftp)(http) |
TXT |
| GSE156221_SE.MATS.ReadsOnTargetAndJunctionCounts.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE156221_differentially_expressed_genes_edgeR.txt.gz |
97.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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