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Series GSE156205 Query DataSets for GSE156205
Status Public on Aug 18, 2020
Title Small RNA sequencing of HCV infected hepatoma cells
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary HCV infection requires both virus and host factors, including endogenous genes, a large proportion of which are noncoding RNAs (ncRNAs). The identification and mechanistic elucidation of these stably and conservatively expressed RNAs will shed light on HCV gene diagnosis and therapy. Nevertheless, most studied ncRNAs in HCV are microRNAs, with numerous other types of ncRNAs being neglected, such as long noncoding RNAs (lncRNAs) or small nucleolar RNAs (snoRNAs). Here, using two different hepatoma cell lines, we performed small RNA sequencing and analyzed the differential expression of microRNAs and snoRNAs, which provide data for further functional validation of snoRNAs and microRNAs during HCV infection.
Overall design Huh7 and Huh7.5.1 cells were infected with JFH-1 HCVcc, and total RNA was extracted at specific time points (0, 6, 24, and 48 h) postinfection using TRIzol reagent (Invitrogen, Carlsbad, USA) according to the manufacturer’s instructions for transcriptome sequencing. For small RNA sequencing, 10 μg of total RNA for each sample was used for small RNA cDNA library preparation, and microRNA and snoRNA were mapped and analyzed.
Contributor(s) Qian X, Xu C, Qi Z
Citation(s) 33042070, 35402303
Submission date Aug 13, 2020
Last update date Apr 19, 2022
Contact name Chen Xu
Phone +86-13774294166
Organization name The Second Military Medical University
Street address No, 800, Rd. Xiangyin
City Shanghai
ZIP/Postal code 200433
Country China
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (8)
GSM4726722 None infected Huh7 cells
GSM4726723 6 hours after HCV infection in Huh7 cells
GSM4726724 24 hours after HCV infection in Huh7 cells
BioProject PRJNA657046
SRA SRP277420

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Supplementary file Size Download File type/resource
GSE156205_All_Counts.txt.gz 49.7 Kb (ftp)(http) TXT
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