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Status |
Public on Aug 13, 2020 |
Title |
ATAC-Seq Data of Adipose Stem Cells from Subcutaneouse Adipose Tissue of Normal-Weight Polycystic Ovary Syndrome Women Undergoing Adipogenesis in vitro |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
In previous studies, multiple animal models (rhesus monkeys, sheep) have suggested a developmental programming effect of PCOS. The goal of this study was to examine whether developmental programming may accompany epigenetic changes by examining intrinsic epigenetic differences, specifically chromatin accessibility, in adipose stem cells (ASCs) from subcutaneous (SC) adipose tissue of PCOS women vs age- and BMI-matched control samples as they mature into adipocytes in vitro.
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Overall design |
ASCs were isolated from SC adipose tissue following SC abdominal biopsy in 3 PCOS women and 3 age and BMI matched controls for RNA-sequencing. Second generation of stem cells were cultured until cells reached confluency and expanded for two additional generations until fourth generation. Fourth generation ASCs were then cultured in adipogenic differentiation medium for 0-12 days and processed for ATAC-seq. Libraries were prepared according to Berostro et al with minor modifications, and visualized on a TapeStation 2200 using D1000 ScreenTape. Libraries were pooled and paired-end sequenced on the Illumina HiSeq4000.
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Contributor(s) |
Leung KL, Davis BA, Okhovat M, Morselli M, Carbone L, Pellegrini M, Chazenbalk GD |
Citation(s) |
33228780 |
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Submission date |
Aug 12, 2020 |
Last update date |
Dec 01, 2020 |
Contact name |
Brett Davis |
E-mail(s) |
davibr@ohsu.edu
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Organization name |
Oregon Health and Science University
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Street address |
3181 SW Sam Jackson Park Rd
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City |
Portland |
State/province |
Oregon |
ZIP/Postal code |
97239 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (18)
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Relations |
BioProject |
PRJNA656827 |
SRA |
SRP277244 |