|
| Status |
Public on Oct 31, 2021 |
| Title |
Intracellular Staphylococcus aureus perturbs the host cell Ca2+-homeostasis to promote cell death |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The pathogen Staphylococcus aureus can invade and survive within human cells. There, the intracellular bacterium induces host cell death, which may facilitate spread of infection and tissue destruction. We here conducted a shRNA screen and infected the library of knock-down cells with S. aureus. We identified calcium signaling pathways to play a role in S. aureus invasion and cytotoxicity. For latter, the intracellular bacteria induce a cytoplasmic and mitochondrial Ca2+-overload, which results in host cell death.
|
| |
|
| Overall design |
HeLa shRNA Screen
|
| |
|
| Contributor(s) |
Stelzner K, Winkler A, Chunguang L, Ade CP, Dandekar T, Fraunholz MJ, Rudel T |
| Citation(s) |
33323513 |
| |
| Submission date |
Aug 12, 2020 |
| Last update date |
Feb 03, 2022 |
| Contact name |
Martin Fraunholz |
| E-mail(s) |
martin.fraunholz@uni-wuerzburg.de
|
| Phone |
+499313183242
|
| Organization name |
University of Wuerzburg
|
| Department |
Chair of Microbiology
|
| Street address |
Theodor-Boveri-Weg
|
| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (30)
|
|
| Relations |
| BioProject |
PRJNA656694 |
| SRA |
SRP277145 |
Less...
More...