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| Status |
Public on Jun 15, 2009 |
| Title |
Uncovering the Arabidopsis thaliana nectary transcriptome |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Many flowering plants attract pollinators by offering a reward of floral nectar. Remarkably, the molecular events involved in the development of nectaries, the organs that produce nectar, as well as the synthesis and secretion of nectar itself, are poorly understood. Indeed, to date, no genes have been shown to directly affect the de novo production or quality of floral nectar. To address this gap in knowledge, the ATH1 Affymetrix GeneChip array was used to systematically investigate the Arabidopsis nectary transcriptome to identify genes and pathways potentially involved in nectar production. In this study, we identified a large number of genes differentially expressed between secretory lateral nectaries and non-secretory median nectary tissues, as well as between mature lateral nectaries (post-anthessis) and immature lateral nectary tissue (pre-anthesis).
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| Overall design |
Three different types of RNA samples were prepared from Arabidopsis thaliana ecotype Columbia-0 nectaries: mature lateral nectaries (MLN; Stage 14-15 flowers), immature lateral nectaries (ILN, Stage 11-12 flowers), and mature median nectaries (MMN, Stage 14-15 flowers) [developmental stages defined by (Smyth et al., 1990)]. MLN are secretory tissues, whereas, ILN and MMN are pre-secretory and nonsecretory tissues, respectively. All nectary tissues were separately dissected by hand from the flowers of primary inflorescences of ca. 30-35 day-old plants. All plants were grown in soil with a 16h light/8h dark light regimen. Due to the small size of nectaries, dissections took place over several days from 4-8 hours after dawn (h.a.d.).
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| Contributor(s) |
Kram BW, Xu WW, Carter CJ |
| Citation(s) |
19604393 |
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| Submission date |
Apr 08, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Clay J Carter |
| E-mail(s) |
cjcarter@d.umn.edu
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| Phone |
218-726-7347
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| Organization name |
University of Minnesota Duluth
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| Department |
Biology
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| Street address |
1035 Kirby Dr
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| City |
Duluth |
| State/province |
MN |
| ZIP/Postal code |
55812 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA115723 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15601_RAW.tar |
27.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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