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| Status |
Public on Jan 08, 2021 |
| Title |
Induction of spontaneous human neocentromere formation and long-term maturation (QuantChIP-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report a chromosome engineering system for human neocentromere formation and characterize the first experimentally generated human neocentromere. Neocentromere formation promotes local H3K9me3 eviction and cohesin and RNA polymerase II recruitment. Long-term culture results in gradual maturation of the inner centromere.
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| Overall design |
Examination of the indicated proteins occupancy before (parental cell line,S40-RPE) and after neocentromere formation (neocentromere cell line,Neo4p13) . Same analysis in Neo4p13 after long-term continuous culture (Neo4p13_100d and 200d). All samples were spiked-in 1% crosslinked immortalized mouse embrionic fibroblats (MEFs) for quantitative analysis.
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| Contributor(s) |
Murillo-Pineda M, Jansen LT |
| Citation(s) |
33443568 |
| |
| Submission date |
Aug 06, 2020 |
| Last update date |
Jan 18, 2021 |
| Contact name |
Marina Murillo-Pineda |
| Organization name |
University of Oxford
|
| Department |
Department of Biochemistry
|
| Lab |
Lars E.T. Jansen Laboratory
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3QU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE155829 |
Induction of spontaneous human neocentromere formation and long-term maturation |
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| Relations |
| BioProject |
PRJNA655721 |
| SRA |
SRP276468 |
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