 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 03, 2020 |
| Title |
A High Docosahexaenoic Acid Diet Alters the Lung Inflammatory Response to Acute Dust Exposure |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Agricultural workers are at risk for the development of acute and chronic lung diseases due to their exposure to organic agricultural dusts. A diet intervention using the omega-3 fatty acid docosahexaenoic acid (DHA) has been shown to be an effective therapeutic approach for alleviating a dust-induced inflammatory response. We thus hypothesized a high-DHA diet would alter the dust-induced inflammatory response through the increased production of specialized pro-resolving mediators (SPMs). Mice were pre-treated with a DHA-rich diet 4 week s before being intranasally challenged with a single dose of an extract made from dust collected from a concentrated swine feeding operation (HDE ). This omega-3-fatty-acid-rich diet led to reduced arachidonic acid levels in the blood, enhanced macrophage recruitment, and increased the production of the DHA-derived SPM Resolvin D1 (RvD1) in the lung following HDE exposure. An assessment of transcript-level changes in the immune response demonstrated significant differences in immune pathway activation and alterations of numerous macrophage-associated genes among HDE-challenged mice fed a high DHA diet. Our data indicate that consuming a DHA-rich diet leads to the enhanced production of SPMs during an acute inflammatory challenge to dust, supporting a role for dietary DHA supplementation as a potential therapeutic strategy for reducing dust-induced lung inflammation.
|
| |
|
| Overall design |
Randomly selected left lung tissues (n = 24) out of the possible 40 mice, representing tissues collected across three unique experimental trials, were homogenized and RNA was extracted using the PureLink RNA Mini Kit (Invitrogen, Carlsbad, California, USA). RNA sample quality was quantified using the NanoDrop ND-100 (NanoDrop Technologies, Inc, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer (UC Riverside Core Facilities, Agilent Technologies, Santa Clara, CA, USA). The assessment of transcript-level gene expression changes was performed using the NanoString mouse Immunology Panel (NanoString Technologies, Seattle, WA, USA), a codeset designed to target 561 genes related to inflammation and the immune response. Fifty to one hundred nanograms of total RNA was mixed with the codeset and reporter probes and hybridized for 16 h to form a purified target–probe complex that was then imaged and quantified with a nCounter Sprint profiler. Gene expression data analysis was performed using the nCounter Analysis System, nSolver 4.0 software. The expression data were normalized by using the geometric mean of 4 housekeeping genes: OAZ1, PPIA, RPL19, and EEF1G. Following normalization, 20 of the samples passed normalization and were viable to use for the subsequent analyses, including 4 control diet + saline, 6 control diet + HDE, 3 DHA diet + saline, and 7 DHA + HDE samples.
|
| |
|
| Contributor(s) |
Nordgren TM, Dominguez EC, Heires AJ, Pavlik J, Larsen TD, Guardado S, Sisson JH, Baack ML, Romberger DJ |
| Citation(s) |
32759853 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 ES025819 |
The role of omega-3 fatty acids and bioactive lipid signaling in airway inflammation and resolution following organic dust exposures |
UNIVERSITY OF CALIFORNIA RIVERSIDE |
Tara M Nordgren |
|
| |
| Submission date |
Aug 02, 2020 |
| Last update date |
Nov 02, 2020 |
| Contact name |
Tara M Nordgren |
| Organization name |
Colorado State University
|
| Department |
Environmental and Radiological Health Sciences
|
| Lab |
Nordgren
|
| Street address |
Colorado State University, Physiology 133
|
| City |
Fort Collins |
| State/province |
CO |
| ZIP/Postal code |
80523 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19964 |
nCounter Mouse Immunology Panel |
|
| Samples (20)
|
| GSM4705898 |
20180810_30102053701118-01_Sample01_01 (Control : Saline) |
| GSM4705899 |
20180810_30102053701118-01_Sample02_02 (Control : Saline) |
| GSM4705900 |
20180810_30102053701118-01_Sample04_04 (Control : HDE) |
| GSM4705901 |
20180810_30102053701118-01_Sample05_05 (Control : HDE) |
| GSM4705902 |
20180810_30102053701118-01_Sample07_07 (DHA : Saline) |
| GSM4705903 |
20180810_30102053701118-01_Sample08_08 (DHA : Saline) |
| GSM4705904 |
20180810_30102053701118-01_Sample09_09 (DHA : HDE) |
| GSM4705905 |
20180810_30102053701118-01_Sample10_10 (DHA : HDE) |
| GSM4705906 |
20180810_30102053701118-01_Sample11_11 (DHA : HDE) |
| GSM4705907 |
20180810_30102053701118-01_Sample12_12 (DHA : HDE) |
| GSM4705908 |
20190725_30102343970221-01_Sample01_01 (Control : Saline) |
| GSM4705909 |
20190725_30102343970221-01_Sample02_02 (Control : Saline) |
| GSM4705910 |
20190725_30102343970221-01_Sample03_03 (Control : HDE) |
| GSM4705911 |
20190725_30102343970221-01_Sample04_04 (Control : HDE) |
| GSM4705912 |
20190725_30102343970221-01_Sample05_05 (Control : HDE) |
| GSM4705913 |
20190725_30102343970221-01_Sample06_06 (Control : HDE) |
| GSM4705914 |
20190725_30102343970221-01_Sample07_07 (DHA : Saline) |
| GSM4705915 |
20190725_30102343970221-01_Sample10_10 (DHA : HDE) |
| GSM4705916 |
20190725_30102343970221-01_Sample11_11 (DHA : HDE) |
| GSM4705917 |
20190725_30102343970221-01_Sample12_12 (DHA : HDE) |
|
| Relations |
| BioProject |
PRJNA650207 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE155539_Figures.tar.gz |
1.9 Mb |
(ftp)(http) |
TAR |
| GSE155539_Normalized_Counts__NanoString_Data.xlsx |
119.5 Kb |
(ftp)(http) |
XLSX |
| GSE155539_Raw_Counts__NanoString_Data.xlsx |
96.0 Kb |
(ftp)(http) |
XLSX |
| Processed data included within Sample table |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...