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| Status |
Public on Jul 31, 2020 |
| Title |
RNA-Seq of UXT KO in Primary Sertoli cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We created a knockout model of ART-27(UXT) specially in the Sertoli cells of the mouse testes. In order to see how loss of ART-27 effects the Sertoli cell transcriptome we isolated Sertoli cells from WT and KO animals. The WT cells were infected with a vector only construct, and the KO cells were infected with CRE producing construct.
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| |
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| Overall design |
examination of transcriptional changes in WT primary Sertoli cells and KO Primary Sertoli cells. Each condition was done in triplicate
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| |
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| Contributor(s) |
Thomas PA, Ruff SE, Ha S, Logan S |
| Citation(s) |
32678429 |
| |
| Submission date |
Jul 30, 2020 |
| Last update date |
Oct 30, 2020 |
| Contact name |
Susan Logan |
| E-mail(s) |
Susan.Logan@nyumc.org, Phillip.Thomas@nyumc.org, Susan.ha@nyumc.org
|
| Organization name |
NYU School of Medicine
|
| Department |
Urology
|
| Lab |
Logan Lab
|
| Street address |
450 East 29th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA649687 |
| SRA |
SRP274268 |
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