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| Status |
Public on Jul 02, 2021 |
| Title |
Single Cell analysis of the most primitive Human Hematopoietic Stem Cell Population. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Single cell transcriptomic profiling (sc RNA-seq) of the most primitive Human Hematopoietic Stem Cell Population described: CD34+CD38-CD45RA-CD49f+EPCR+ (hereafter EPCR+).
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| Overall design |
Purpose: to search for potential heterogeneity within EPCR+ HSCs at the single cell level.
Methods: single cells (from human cord-blood CD34+ cells) were flow-sorted fisrt then loaded into C1 Fludigm chips: 3 independent sorts; 4chips in total were used.
Multiplexed Nextera XT was used to generate libraries. Each single cell was barcoded.
Conclusions: this study supports that this population is highly homogeneous at the transcriptional level.
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| Contributor(s) |
Anjos-Afonso F, Buettner F, Bonnet D |
| Citation(s) |
35440586 |
| |
| Submission date |
Jul 27, 2020 |
| Last update date |
Jan 26, 2024 |
| Contact name |
Florian Buettner |
| Organization name |
DKFZ
|
| Department |
DKTK Frankfurt
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| Street address |
Theodor-Stern-Kai 7
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| City |
Frankfurt |
| State/province |
Germany |
| ZIP/Postal code |
60590 |
| Country |
Germany |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (183)
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| Relations |
| BioProject |
PRJNA648921 |
| SRA |
SRP273721 |
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