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| Status |
Public on Jul 25, 2020 |
| Title |
Brassica oleracea-Alternaria brassicicola interaction during the early stages of leaf infection |
| Platform organism |
Arabidopsis thaliana |
| Sample organism |
Brassica oleracea var. capitata |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression changes during the initial stages of black spot disease caused by Alternaria brassicicola on Brassica oleracea (Brassica oleracea var. capitata f. alba, white cabbage) leaves were investigated with Arabidopsis thaliana oligonucleotide microarrays. Transcriptional profiling of infected B. oleracea leaves revealed that photosynthesis was the most negatively regulated biological process. The negative regulation of 6 photosynthesis-related genes, mainly the genes involved in the photosynthesis light reaction and Calvin cycle, was observed as early as 12 hours post infection (hpi). It progressed through 48-hpi stage, when 44 down-regulated photosynthesis-related genes were detected. The analyses of infected leaves at microscopic, ultrastructural and physiological levels supported the microarray-based observations and indicated that the photosynthetic processes are suppressed in B. oleracea as a result of the fungal infection.
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| Overall design |
Time-course experiment, two-color, common reference design, involving two biological replicates for each time point. Samples represent plant leaves harvested before infection (0hpi) and at different time points post infection with fungus (12h, 24h and 48h). Each biological replicate sample was prepared from leaves harvested from three plants. Cy-5 labeled samples for each time point and each replicate were individually hybridized to Cy3-labeled common reference (representing pooled RNA samples from mock-treated plants, harvested at the same time points as infected plants), on a total of 8 microarrays.
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| Contributor(s) |
Zmienko A, Macioszek V |
| Citation(s) |
33092216 |
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| Submission date |
Jul 24, 2020 |
| Last update date |
Oct 25, 2020 |
| Contact name |
Agnieszka Zmienko |
| E-mail(s) |
akisiel@ibch.poznan.pl
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| Organization name |
Institute of Bioorganic Chemistry PAS
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| Street address |
Noskowskiego 12/14
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| City |
Poznan |
| ZIP/Postal code |
61-704 |
| Country |
Poland |
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| Platforms (1) |
| GPL7725 |
Galbraith Laboratory Operon Arabidopsis thaliana 31.2K Long Oligonucleotide Microarray version 3.0 (ATv307.04.Z) |
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| Samples (8)
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| GSM4694449 |
Infected leaf, 12 hpi, replicate 2 |
| GSM4694450 |
Infected leaf, 24 hpi, replicate 1 |
| GSM4694451 |
Infected leaf, 24 hpi, replicate 2 |
| GSM4694452 |
Infected leaf, 48 hpi, replicate 1 |
| GSM4694453 |
Infected leaf, 48 hpi, replicate 2 |
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| Relations |
| BioProject |
PRJNA648351 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE155051_RAW.tar |
25.0 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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