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| Status |
Public on Jul 22, 2020 |
| Title |
Age-related transcriptional profiling of phagocytic cells isolated from murine epididymal white adipose tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Age-related changes in macrophages are thought to underlie deleterious changes in adipose tissue during aging. RNAseq analysis of phagocyte-enriched and -depleted stromal vascular fractions of perigonadal white adipose tissue from young and aged mice revealed age-related differences in gene expression towards identification of adipose tissue macrophage-specific regulation.
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| Overall design |
The stromal vascular fractions (SVF) of pooled epididymal white adipose tissue (eWAT) were isolated from young (10-week old; n=12) and old (80-week old; n=6) C57BL/6 mice and incubated with a suspension of superparamagnetic nanoparticles (~150-nm EasySep™ Magnetic Particles; StemCell Technologies). Subsequently, cells that phagocytized these particles were separated from non-phagocytic cells by performing magnetic-activated cell isolation, yielding macropahge-enriched phagocytic fraction and resultant phagocyte-depleted sub-populations. These fractions were used for RNAseq analysis.
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| Contributor(s) |
Gudkov AV, Hall BM |
| Citation(s) |
32856419 |
| |
| Submission date |
Jul 21, 2020 |
| Last update date |
Oct 21, 2020 |
| Contact name |
Brandon Hall |
| E-mail(s) |
bhall@genomeprotection.com
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| Organization name |
Everon Biosciences, Inc.
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| Street address |
640 Ellicott Street, Ste. 444
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| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14203 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA647785 |
| SRA |
SRP273120 |
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