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| Status |
Public on Jan 11, 2021 |
| Title |
Crosstalk between Three Fungus-specific Transcription Factors, WetA, VosA, and VelB, in Asexual Spores of Aspergillus nidulans [ChIP-seq] |
| Organism |
Aspergillus nidulans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In filamentous fungi, asexual sporulation involves morphological differentiation and metabolic changes. The process of asexual spore formation is tightly regulated by a variety of transcription factors including VosA, VelB, and WetA. A number of studies have demonstrated that these three transcription factors are key regulators of asexual spore formation and maturation in the model filamentous fungus Aspergillus nidulans. To gain a more mechanistic view of the roles these transcription factors play in asexual spores, genome-wide and metabolomic analyses were conducted in A. nidulans conidia. RNA sequencing and chromatin immunoprecipitation-based sequencing data suggested that the three transcription factors directly or indirectly regulate the expression of genes associated with spore-wall integrity, asexual development, and secondary metabolism. In addition, metabolomics analysis of conidia extracts showed strikingly different primary and secondary metabolite profiles for wild-type and mutant conidia. These results suggest that WetA, VosA, and VelB play key roles in the morphological development of and metabolic changes in conidia. This entry is for the ChIP-seq data.
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| Overall design |
To identify the VelB direct target genes and compare the putative direct target genes of VosA and VelB, ChIP experiments, followed by high-throughput sequencing of the enriched DNA fragments in the conidia from the WT strain and strains containing VosA-FLAG or VelB-FLAG conidia, were carried out. A commercially available anti-FLAG antibody-Dynabead complex was used. WT samples (that did not contain the FLAG epitope) were compared to samples from strains that contained the FLAG epitope on the proteins of interest. Separate WT samples, prepared using the same methods, were analyzed for VosA-FLAG and VelB-FLAG experiments. Six replicates were analyzed the VosA-FLAG samples. Three replicates were analyzed for all other samples.
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| Contributor(s) |
Wu M, Mead ME, Lee M, Neuhaus GF, Adpressa DA, Martien JI, Son Y, Moon H, Amador-Noguez D, Han K, Rokas A, Loesgen S, Yu J, Park H |
| Citation(s) |
33563821 |
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| Submission date |
Jul 17, 2020 |
| Last update date |
Feb 22, 2021 |
| Contact name |
Antonis Rokas |
| Organization name |
Vanderbilt University
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| Department |
Biological Sciences
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| Street address |
VU Station B 35-1364
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| City |
Nashville |
| ZIP/Postal code |
37235 |
| Country |
USA |
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| Platforms (1) |
| GPL20839 |
Illumina HiSeq 2500 (Aspergillus nidulans) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE154640 |
Crosstalk between Three Fungus-specific Transcription Factors, WetA, VosA, and VelB, in Asexual Spores of Aspergillus nidulans |
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| Relations |
| BioProject |
PRJNA646900 |
| SRA |
SRP272360 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE154630_VelB_peaks.narrowPeak.gz |
73.1 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE154630_VosA_peaks.narrowPeak.gz |
38.2 Kb |
(ftp)(http) |
NARROWPEAK |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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