|
| Status |
Public on Sep 24, 2020 |
| Title |
Single cell RNAseq reveal covid19 patient immune response |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Eighteen patients admitted to CSMC and diagnosed with COVID19 by RT-PCR were stratified into COVID19 mild/moderate, severe, and recovery groups (n=5-6/group). Venous blood was collected into EDTA coated tubes and centrifuged to separate plasma and buffy coat. Plasma was collected and frozen at -80C, and the buffy coat was collected into cryo-preservation media and frozen at -80C. Recovered cells are sorted for live-dead staining, then fixed with methanol. Fixed single cells were further captured using 10x chromium Next GEM 3 prime v3.1 kit. Two patients samples from the same group were mixed, captured and sequenced together.
|
| |
|
| Overall design |
single-cell RNA-seq of blood buffy coat from COVID-19 patients.
|
| |
|
| Contributor(s) |
Yao C, Chen P |
| Citation(s) |
32743611, 33357411 |
| |
| Submission date |
Jul 16, 2020 |
| Last update date |
May 04, 2021 |
| Contact name |
Changfu Yao |
| E-mail(s) |
changfu.yao@cshs.org
|
| Phone |
424-315-4333
|
| Organization name |
cedars-sinai medical center
|
| Department |
Lung and Regenerative Medicine Institutes
|
| Lab |
Stripp Lab
|
| Street address |
8700 Beverly Blvd. AHSP Room A9401
|
| City |
los angeles |
| State/province |
Ca |
| ZIP/Postal code |
90048 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA646704 |
| SRA |
SRP272244 |
Less...
More...