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Series GSE154407 Query DataSets for GSE154407
Status Public on Sep 29, 2021
Title Robustness in the feedback control of the retinoic acid network response to environmental disturbances (BioMark-qRT-PCR)
Organism Xenopus laevis
Experiment type Expression profiling by RT-PCR
Summary Retinoic acid (RA) is a central developmental signal whose perturbation results in teratogenic outcomes. RA is tightly regulated during early embryonic development to achieve fine-tuning of the instructive and permissive signals regulating multiple developmental processes including head formation. We aim to identify the key mechanisms underlying the homeostatic regulation and tuning of the retinoic acid signaling levels, the disruption of which in the head lead to wide ranging developmental malformations including microcephaly and other craniofacial defects. We performed transient physiological RA signaling disturbances during Xenopus laevis embryogenesis followed by kinetic gene expression analysis of the recovery at multiple time points.
 
Overall design We considered multiple experimental perturbations including exogenous addition of 10 nM RA, inhibition of RA production by 50 uM DEAB (an inhibitor of RALDH2 enzyme) and no exposure control. We obtained data from six independent clutches for each experimental condition. The embryos were isolated at multiple time points (t=0, 1.5, 3, and 4.5 hours) following two hours of perturbation and a wash.
 
Contributor(s) Parihar M, Bendelac-Kapon L, Gur M, Belorkar A, Achanta S, Kinberg K, Vadigepalli R, Fainsod A
Citation(s) 34746144
Submission date Jul 14, 2020
Last update date Nov 10, 2021
Contact name Rajanikanth Vadigepalli
E-mail(s) rajanikanth.vadigepalli@jefferson.edu
Organization name THOMAS JEFFERSON UNIVERSITY
Street address 1020 LOCUST ST RM 314
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
 
Platforms (1)
GPL28865 Fluidigm BioMark-qRT-PCR (Xenopus laevis)
Samples (72)
GSM4670669 G_control_t_0hr
GSM4670670 G_control_t_1.5hr
GSM4670671 G_control_t_3hr
This SubSeries is part of SuperSeries:
GSE154408 Asymmetric robustness in the feedback control of the retinoic acid network response to environmental disturbances
Relations
BioProject PRJNA646207

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154407_relative_gene_expression.xlsx 34.9 Kb (ftp)(http) XLSX
Processed data are available on Series record

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