|
| Status |
Public on Sep 13, 2020 |
| Title |
Effects of PAK4 knockdown on gene expression in glioblastoma-associated endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
PAK4 was knocked down in human glioblastoma (GBM)-associated endothelial cells (ECs) using CRISPR-Cas9/gRNA. RNA was isolated and analyzed by RNAseq. The effects of PAK4 knockout on gene expression were determined.
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| |
|
| Overall design |
ECs were isolated from GBM tumors and transduced with the lentivirus expressing PAK4 sgRNA or control sgRNA, followed by cell selection by incubation with medium containing 2 µg/ml puromycin. RNA was isolated and analyzed by RNAseq.
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| |
|
| Contributor(s) |
Ma W, Zhang D, Fan Y |
| Citation(s) |
35121889 |
| |
| Submission date |
Jul 09, 2020 |
| Last update date |
May 11, 2023 |
| Contact name |
Yi Fan |
| E-mail(s) |
Yi.Fan@pennmedicine.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Radiation Oncology
|
| Lab |
Fan Lab
|
| Street address |
3400 Civic Center Blvd, SCTR 8-168
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA645173 |
| SRA |
SRP271151 |
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