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| Status |
Public on Sep 15, 2020 |
| Title |
Use of the Illumina EPIC methylation array for epigenomic research in the crab-eating macaque (Macaca fascicularis) |
| Platform organism |
Homo sapiens |
| Sample organism |
Macaca fascicularis |
| Experiment type |
Methylation profiling by array
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| Summary |
Among the 865,918 probes in the Illumina DNA methylation arrays MethylationEPIC BeadChip, a total of 183,509 probes (21.2%) were selected as high-confidence array probes in the crab-eating macaque. Subsequent comparisons revealed that the data from these probes showed good concordance with other DNA methylation datasets of the crab-eating macaque. The selected high-confidence array probes would be useful for high-throughput DNA methylation assays of the crab-eating macaque.
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| Overall design |
Through bioinformatic analyses involving cross-species comparison and consideration of probe performance, we selected array probes that can be reliably used for the crab-eating macaque genome. A DNA methylation assay using an EPIC array was performed on genomic DNA extracted from the brains of five crab-eating macaques. The obtained DNA methylation data were compared with a publicly available dataset.
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| Contributor(s) |
Nakachi Y, Ishii K, Bundo M, Masuda T, Iwamoto K |
| Citation(s) |
33037870 |
| |
| Submission date |
Jul 09, 2020 |
| Last update date |
Oct 12, 2020 |
| Contact name |
Kazuya Iwamoto |
| E-mail(s) |
iwamotok@kumamoto-u.ac.jp
|
| Phone |
+81-96-373-5054
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| Organization name |
Kumamoto University
|
| Department |
Faculty of Life Sciences
|
| Lab |
Department of Molecular Brain Science
|
| Street address |
1-1-1 Honjo, Chuo-ku
|
| City |
Kumamoto |
| ZIP/Postal code |
860-8556 |
| Country |
Japan |
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| Platforms (1) |
| GPL23976 |
Illumina Infinium HumanMethylation850 BeadChip |
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| Samples (5)
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| GSM4663614 |
genomic DNA from crab-eating macaque brain; control-1 |
| GSM4663615 |
genomic DNA from crab-eating macaque brain; control-2 |
| GSM4663616 |
genomic DNA from crab-eating macaque brain; control-3 |
| GSM4663617 |
genomic DNA from crab-eating macaque brain; control-4 |
| GSM4663618 |
genomic DNA from crab-eating macaque brain; control-5 |
|
| Relations |
| BioProject |
PRJNA645077 |
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