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| Status |
Public on Jul 07, 2020 |
| Title |
Aberrantly expressed lncRNAs and mRNAs of osteogenically differentiated mesenchymal stem cells in ossification of the posterior longitudinal ligament |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
Ectopic bone formation is the chief characteristic of ossification of the posterior longitudinal ligament (OPLL). Emerging evidence has revealed that long non-coding RNAs (lncRNAs) can regulate the osteogenic differentiation of mesenchymal stem cells (MSCs), which are the main cells responsible for bone formation. However, the role of lncRNAs in the pathogenesis of OPLL remains unclear. In this study, 725 aberrantly expressed lncRNAs and 664 mRNAs in osteogenically differentiated MSCs from OPLL patients (OPLL MSCs) were identified by microarrays and confirmed by qRT-PCR assays. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that the most enriched pathways included the p53, JAK-STAT and PI3K-Akt signaling pathways. The coexpression network showed the interactions between the aberrantly expressed lncRNAs and mRNAs in OPLL MSCs, and the potential targets and transcription factors of the lncRNAs were predicted. Our research demonstrated the aberrantly expressed lncRNA and mRNA and the potential regulatory networks involved in the ectopic bone formation of OPLL. These findings imply that lncRNAs may play a vital role in OPLL, which provides a new perspective on the pathogenesis of OPLL.
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| Overall design |
The control group including three HDMSC samples was derived from three healthy donors and the experimental group including three OPLLMSC samples was derived from three OPLL patients. The cells used for microarray analysis were cultured in osteogenic differentiation medium for 10 days.
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| Contributor(s) |
Cai Z, Liu W |
| Citation missing |
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| Submission date |
Jul 06, 2020 |
| Last update date |
Jul 10, 2020 |
| Contact name |
Zhaopeng Cai |
| Organization name |
Sun Yat-sen University
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| Street address |
No. 3025, Shennan Middle Road, Futian District
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| City |
Shenzhen |
| ZIP/Postal code |
518033 |
| Country |
China |
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| Platforms (1) |
| GPL20115 |
Agilent-067406 Human CBC lncRNA + mRNA microarray V4.0 (Probe name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA644325 |
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