|
| Status |
Public on Jun 28, 2020 |
| Title |
HuD interacting circular RNAs in the mouse striatum |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
This study explores whether HuD could bind to and regulate the expression circRNAs from genes associated with neuronal development and synaptic plasticity
circRNAs bound to HuD were isolated from the striatum of HuD-OE mice by RNA immunoprecipitation (RIP) as described in Bolognani et al, 2010 (https://doi.org/10.1093/nar/gkp863) using Dynabeads® (Thermo Fisher Scientific) coated with mouse monoclonal anti-myc tag antibody (9B11; Cell Signalling Technology Inc.) specifically recognizing myc-tagged HuD transgenic protein expressed in HuD-OE as described before (Bolognani et al., 2010; Zimmerman et al., 2020). Controls for RIP assays were performed using either non-immune IgG and HuD-OE tissue or the myc-tag antibody and wild type (WT) tissue.
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| |
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| Overall design |
RIP assay were run in triplicates using 3 HuD transgenic and 3 wildtype mice
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| |
|
| Contributor(s) |
Dell'Orco M, Perrone-Bizzozero N |
| Citation(s) |
32849796 |
| |
| Submission date |
Jun 27, 2020 |
| Last update date |
Aug 31, 2020 |
| Contact name |
Nora Perrone-Bizzozero |
| E-mail(s) |
nbizzozero@salud.unm.edu
|
| Organization name |
University of New Mexico
|
| Department |
Neurosciences
|
| Lab |
NPB
|
| Street address |
1 University of New Mexico MSC08 4740
|
| City |
Albuquerque |
| State/province |
NM |
| ZIP/Postal code |
87131 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21826 |
074663 Arraystar Mouse CircRNA microarray V2 |
|
| Samples (9)
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|
| Relations |
| BioProject |
PRJNA642303 |
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