|
| Status |
Public on Jul 17, 2020 |
| Title |
Genome-wide profiling for H2A.Z and genome accessibility using ATACseq in Uhrf1hepKO mouse livers compared to WT livers |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
This study was carried out to investigate potential changes in the genome-wide distribution of H2AZ and genome accessibility using ATACseq in livers where Uhrf1 is deleted in hepatocytes (Uhrf1-fl/fl;Alb:Cre) compared to controls (Uhrf1-fl/fl).
|
| |
|
| Overall design |
ChIP was carried out to pull down H2AZ bound chromatin from nuclei isolated from s single Uhrf1-Fl/fl liver and a single (Uhrf1-fl/fl;Alb:Cre) male liver between 8-12 weeks of age. This chromatin fraction was then digested using micrococcal nuclease to release mononucleosomes. DNA fragments purified from nucleosomes were then used to generate libraries and sequenced. The same livers were used for chromatin
accessibility using the ATAC-seq Protocol as described in Buenrostro et al (2015).
|
| |
|
| Contributor(s) |
Wang S, Zhang C, Sadler (Edepli) KC, Madakashira B, Magnani E |
| Citation(s) |
34226551 |
| |
| Submission date |
Jun 23, 2020 |
| Last update date |
Nov 04, 2021 |
| Contact name |
Kirsten Sadler Edepli |
| E-mail(s) |
kirsten.edepli@nyu.edu
|
| Phone |
971568327587
|
| Organization name |
New York University Abu Dhabi
|
| Department |
Biology
|
| Street address |
PO Box 129188
|
| City |
Abu Dhabi |
| State/province |
Abu Dhabi |
| ZIP/Postal code |
000 |
| Country |
United Arab Emirates |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA641416 |
| SRA |
SRP268507 |
Less...
More...