|
| Status |
Public on Dec 31, 2021 |
| Title |
Early life stress leads to gene expression down-regulation in prefrontal cortex [RNA-seq] |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Early life stress (ELS) is associated with adverse mental health outcomes including anxiety, depression and addiction-like behaviours. While ELS is known to affect the developing brain by leading to increased stress responsiveness and increased glucocorticoid levels, the molecular mechanisms underlying the detrimental effects of ELS remain incompletely characterised. Rodent models have been instrumental in beginning to uncover the molecular and cellular underpinnings of ELS. Limited nesting (LN), an ELS behavioural paradigm with significant improvements over maternal separation, mimics human maternal neglect. We have previously shown that LN leads to anxiety like-behaviours in rats. Here we assessed gene expression changes induced by ELS in rat prefrontal cortex by RNA-sequencing. We show that LN leads primarily to transcriptional repression and identify a molecular signature of LN in rat PFC that is robust to the behavioural paradigm and replicable across rodent species (mouse and rat).
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| |
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| Overall design |
RNA-seq from prefrontal cortex of 5 control and 5 early life stress rats
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| |
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| Contributor(s) |
Green N, Maniam J, Riese J, Morris M, Voineagu I |
| Citation(s) |
33796639 |
| |
| Submission date |
Jun 23, 2020 |
| Last update date |
Nov 06, 2022 |
| Contact name |
Irina Voineagu |
| Organization name |
The University of New South Wales
|
| Department |
Biotechnology and Biomolecular Sciences
|
| Street address |
High St
|
| City |
Kensington |
| State/province |
NSW |
| ZIP/Postal code |
2052 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
|
| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE153044 |
Early life stress leads to gene expression down-regulation in prefrontal cortex |
|
| Relations |
| BioProject |
PRJNA641512 |
| SRA |
SRP268669 |
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