|
| Status |
Public on Feb 18, 2021 |
| Title |
10x Genomics single-cell RNA-seq of isolated Adult ID4-EGFP-bright mouse spermatogonia treated with Rapamycin |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To reveal distinct transcriptome changes among ID4-EGFP-bright adult mouse spermatogonia associated with mTORC1 activity, single-cell transcriptomes were generated from GFP-bright/CD9-bright spermatogonia from adult mice in three groups: control (untreated), 2 days of Rapamycin treatment (Rapamycin) and 2 days Rapamycin plus 1 day washout (Rapamycin_Release). Based on transplantation studies performed previously, ID4-EGFPbright cells are highly enriched for SSCs. We used the 10x Genomics Chromium to perform single-cell RNA-seq.
|
| |
|
| Overall design |
Each cell population (treatment group) was generated from pooled cells from three adult males.
|
| |
|
| Contributor(s) |
Hermann BP, Suzuki S, McCarrey JR |
| Citation(s) |
33596419 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HD090007 |
Origin and functional significance of the spermatogonial stem cell transcriptome barcode |
THE UNIVERSITY OF TEXAS AT SAN ANTONIO |
Brian Peter Hermann |
|
| |
| Submission date |
Jun 22, 2020 |
| Last update date |
Feb 21, 2021 |
| Contact name |
Brian P. Hermann |
| E-mail(s) |
Brian.Hermann@utsa.edu
|
| Phone |
210-458-8047
|
| Organization name |
University of Texas at San Antonio
|
| Department |
Department of Biology
|
| Street address |
1 UTSA Circle
|
| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78249 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA641008 |
| SRA |
SRP268252 |
