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| Status |
Public on Nov 03, 2009 |
| Title |
Analysis of fetal livers from male mice after protein-restricted diet in mothers |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Prenatal nutrition as influenced by nutritional status of the mother has been identified as a determinant of adult disease. Feeding low-protein diets during pregnancy in rodents is a well-established model to induce “programming” events in offspring. We hypothesized that protein restriction would influence fetal lipid metabolism by inducing epigenetic adaptations.
Methodology/Principal Findings: Pregnant C57BL/6J OlaHsd mice were exposed to a protein restriction protocol (9% vs. 18% casein). Shortly before birth, dams and fetuses were sacrificed. To identify putative epigenetic changes, CpG island methylation microarrays were performed on DNA isolated from fetal livers.
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| Overall design |
10 fetal livers: 5 mice from protein-restriction group (mice 1-5, labeled with Alexa 647) and 5 mice from control group (mice 6-10, labeled with Alexa 555).
5 arrays were used for the mice combinations 1/6, 2/7, 3/8, 4/9, 5/10.
All arrays were run at the UHN Microarray Centre, Toronto.
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| Citation(s) |
19889862 |
| Submission date |
Mar 18, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
vincent bloks |
| Organization name |
UMCG
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| Department |
Pediatrics
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| Lab |
Pediatrics
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| Street address |
Hanzeplein1
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| City |
Groningen |
| ZIP/Postal code |
9713GZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL8311 |
MCGI4.6Kv1_CPG_Mouse_Array_UMCG |
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| Samples (5)
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| GSM381575 |
fetal liver after protein restriction, mice 1 and 6 |
| GSM381576 |
fetal liver after protein restriction, mice 2 and 7 |
| GSM381577 |
fetal liver after protein restriction, mice 3 and 8 |
| GSM381578 |
fetal liver after protein restriction, mice 4 and 9 |
| GSM381579 |
fetal liver after protein restriction, mice 5 and 10 |
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| Relations |
| BioProject |
PRJNA116505 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15280_RAW.tar |
1.8 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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