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| Status |
Public on Dec 01, 2020 |
| Title |
The TAZ-CAMTA1 and YAP-TFE3 fusion proteins modulate the basal TAZ/YAP transcriptional program by recruiting the Ada2a-containing histone acetyltransferase complex [rnaseq_sw872] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The WWTR1(TAZ)-CAMTA1 and YAP1-TFE3 gene fusions are disease defining gene alterations for epithelioid hemangioendothelioma, a vascular cancer. The resultant fusion proteins fuse the C terminus of CAMTA1 and TFE3 in frame to the N terminus of TAZ and YAP, respectively. An unbiased BioID-mass spectrometry/RNAi screen identified YEATS2 and ZZZ3 as components of the Ada2a-containing histone acetyltransferase complex and key interactors of both TAZ-CAMTA1 and YAP-TFE3.
An integrative NGS approach including RNA-Seq, ChIP-Seq, and ATAC-Seq showed TAZ-CAMTA1 and YAP-TFE3 transcriptional programs overlap with TAZ and YAP but also drive expression of a novel transcriptome.
The altered transcriptional program of the fusion proteins owing to altered DNA binding as well as shifts in the chromatin landscape.
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| Overall design |
mRNA profiles of SW872 liposarcoma cells transduced with fusion gene constructs were generated by deep sequencing, in triplicate, using Illumina HiSeq4000.
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| Contributor(s) |
Tanas M, Chimenti M |
| Citation(s) |
33913810 |
| |
| Submission date |
Jun 18, 2020 |
| Last update date |
Jun 03, 2021 |
| Contact name |
Michael S Chimenti |
| E-mail(s) |
michael-chimenti@uiowa.edu
|
| Organization name |
University of Iowa
|
| Department |
Iowa Institute of Human Genetics
|
| Street address |
431 Newton Road
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52245 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE152780 |
The TAZ-CAMTA1 and YAP-TFE3 fusion proteins modulate the basal TAZ/YAP transcriptional program by recruiting the Ada2a-containing histone acetyltransferase complex |
|
| Relations |
| BioProject |
PRJNA640301 |
| SRA |
SRP267807 |
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