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| Status |
Public on Jun 13, 2020 |
| Title |
Gene expression profile of TPT1 gene knock downed human ES-NSC |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To evaluate the function of TPT1 in human ES-derived neural stem cells (ES-NSCs), we knock downed TPT1 gene expression based on the lentivirus system expressing shTPT1 RNA with control. We isolated RNA from ES-NSCs 2 days after infection and subjected to RNA-seq analysis. In silico data analysis supported the function of TPT1 related to cell cycle regulation, consistent with in vitro experimental results.
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| Overall design |
Examination of gene expression profile of TPT1 gene knock downed human ES-NSC
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| Contributor(s) |
Ohta S |
| Citation missing |
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| |
| Submission date |
Jun 12, 2020 |
| Last update date |
Jun 15, 2020 |
| Contact name |
Shigeki Ohta |
| E-mail(s) |
shiohta@keio.jp
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| Organization name |
Keio University
|
| Street address |
35
|
| City |
Shinanomachi,Shinjukuku |
| State/province |
Tokyo |
| ZIP/Postal code |
1658582 |
| Country |
Japan |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA639041 |
| SRA |
SRP267033 |
