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| Status |
Public on Oct 29, 2020 |
| Title |
Cardiomyocytes Infected by SARS-CoV-2 Recruit Cardiotoxic Macrophages [bulk RNA-seq dataset) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Heart injury has been reported in up to 20% of COVID-19 patients, yet the cause of myocardial histopathology is unknown. We examined heart tissue from autopsies of healthy and COVID-19 patients using RNA-seq and identified a strikingly increased CCL2 expression and macrophage population in COVID-19 heart sample. Transwell assays using hESC-derived cardiomyocytes (CMs) and macrophages revealed that SARS-CoV-2 infected CMs secrete CCL2, which recruits macrophages, and this was validated using adult human CMs. Macrophages recruited by CCL2 from infected CMs secrete IL-6 and TNF-α, which causes increased ROS and cell apoptosis of CMs. Finally, a high content chemical screen using FDA-approved drugs identified ranolozine and tofacitinib, which rescue SARS-CoV-2 infected CMs from macrophages-induced cardiotoxicity.
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| |
|
| Overall design |
Bulk RNA-seq of hESC-derived cardiomyocytes, adult human cardiomyocytes, and macrophages with mock or SARS-CoV-2 infection.
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| |
|
| Contributor(s) |
Yang L, Han Y, Chen S |
| Citation(s) |
33236003 |
| |
| Submission date |
Jun 05, 2020 |
| Last update date |
Dec 01, 2020 |
| Contact name |
Shuibing Chen |
| E-mail(s) |
shuibing.chen@gmail.com
|
| Phone |
2127465431
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Surgery
|
| Street address |
A 827B, 1300 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (18)
|
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| This SubSeries is part of SuperSeries: |
| GSE151880 |
Cardiomyocytes Infected by SARS-CoV-2 Recruit Cardiotoxic Macrophages |
|
| Relations |
| BioProject |
PRJNA637580 |
| SRA |
SRP266130 |
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