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| Status |
Public on Feb 01, 2021 |
| Title |
Whole genome bisulfite sequencing from primate brains (orthologous region to human BA46) |
| Organisms |
Macaca mulatta; Pan troglodytes |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We carried out cell-type specific whole genome bisulfite sequencing from primate brains (orthologous region to human BA46) that underwent fluorescence-activated nuclei sorting (FANS).
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| Overall design |
We compared the cell-type specific methylome profiling of sorted nuclei from humans to non-human primates. We analyzed genome-wide DNA methylation levels in adult human Brodmann area 46 (BA46, 27 individuals, GSE108066), and the homologous regions of chimpanzee (11 individuals), and rhesus macaque (15 individuals). Cell-type specific methylome data was obtained using fluorescence-activated nuclei sorting (FANS) with antibodies to either NeuN or OLIG2 to isolate neurons (NeuN) or oligodendrocytes (OLIG2) and their precursors, respectively.
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| Contributor(s) |
Yi SV |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 MH103517 |
Human Specific Brain DNA Methylation and Neuropsychiatric Diseases |
GEORGIA TECH RESEARCH CORPORATION |
SOOJIN YI |
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| |
| Submission date |
Jun 04, 2020 |
| Last update date |
Feb 03, 2021 |
| Contact name |
Soojin V Yi |
| E-mail(s) |
soojin.yi@biology.gatech.edu
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| Organization name |
Georgia Institute of Technology
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| Department |
Biological Sciences
|
| Street address |
950 Atlantic Dr
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30332-0321 |
| Country |
USA |
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| Platforms (2) |
| GPL19129 |
Illumina HiSeq 2500 (Macaca mulatta) |
| GPL19148 |
Illumina HiSeq 2500 (Pan troglodytes) |
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| Samples (50)
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| Relations |
| BioProject |
PRJNA637217 |
| SRA |
SRP265909 |
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