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| Status |
Public on Dec 31, 2021 |
| Title |
RIP-Seq analysis of non-PPR editing factors OZ1, ORRM1, RIP9/MORF9 |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We provide sequences of maize chlroplast RNAs associated with non-PPR editing factors through immunoprecipitation. The accumulated sequences indicate expansive role in RNA processing and potential associations of OZ1-ORRM1 complexes with the ribosome and less efficiently translated mRNAs.
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| Overall design |
Three specific purified polyclonal antibodies were bound to magnetic protein A/G beads. Beads were then suspended in maize chloroplast extracts and RNAs isolated and sequenced after washing.
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| Contributor(s) |
Santibanez PI, Hayes ML, Ngo V |
| Citation missing |
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| |
| Submission date |
Jun 03, 2020 |
| Last update date |
Dec 31, 2021 |
| Contact name |
Michael Lloyd Hayes |
| E-mail(s) |
mhayes3@calstatela.edu
|
| Organization name |
California State University Los Angeles
|
| Department |
Chemistry and Biochemistry
|
| Street address |
5151 State University Dr
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90032 |
| Country |
USA |
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| Platforms (1) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA637019 |
| SRA |
SRP265790 |
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