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Series GSE151658 Query DataSets for GSE151658
Status Public on Jun 03, 2020
Title The orchestrated cellular and molecular responses of the kidney to endotoxin define a precise sepsis timeline
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Utilizing single cell RNA sequencing, we demonstrated multiple time-anchored, cell-type specific events that are key to understand clinical features of sepsis.
 
Overall design Wild type mouse kidneys were harvested and used for single cell RNAseq to examine baseline gene expression patterns across 0,1,4,16,27,36, and 48 hour timepoints after endotoxin (LPS) administration.
Library kit used for this dataset: Chromium Single Cell 3' Reagent Kits V2 was used with read1 L=26 and read2 L=91 configuration.
 
Contributor(s) Janosevic D, Hato T, McCarthy T
Citation(s) 33448928
Submission date Jun 02, 2020
Last update date Mar 16, 2023
Contact name Takashi Hato
Organization name Indiana University School of Medicine
Department Nephrology and Hypertension
Lab Hato/Dagher Lab
Street address 950 W Walnut Street
City Indianapolis
State/province IN
ZIP/Postal code 46202
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (7)
GSM4587730 Control (LPS0hr)_scRNAseq
GSM4587731 LPS_1h (LPS1hr)_scRNAseq
GSM4587732 LPS_4h (LPS4hr)_scRNAseq
Relations
BioProject PRJNA636783
SRA SRP265634

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151658_RAW.tar 260.5 Mb (http)(custom) TAR (of TAR)
GSE151658_immune.all.RDS.gz 82.4 Mb (ftp)(http) RDS
GSE151658_integrated.0h.1h_4h_16h_27h_36h_48h.rds.gz 1.4 Gb (ftp)(http) RDS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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