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Series GSE15156 Query DataSets for GSE15156
Status Public on Apr 22, 2009
Title Gene expression analysis of HPV-immortalized keratinocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify early processes in carcinogenesis, we used an in vitro model, based on the initiating event in cervical cancer, human papillomavirus (HPV) transformation of keratinocytes. We compared gene expression in primary keratinocytes (K) and HPV16-transformed keratinocytes from early (E) and late (L) passages, and from benzo[a]pyrene treated L cells (BP). The transformed cells exhibit similar transcriptional changes to clinical cervical carcinoma. We revealed a contraction in expression of the apoptotic network during HF1 cell transformation, which affected the ability of L and BP cells to execute apoptosis, but did not lead to resistance to apoptotic stimuli. The contraction in the apoptotic machinery during the process of transformation was accompanied by a switch from apoptosis to necrosis in response to CDDP. The shrinkage of the pro- and anti-apoptotic networks appears to be part of a general contraction in the number of genes transcribed in L and BP cells. We also identified a large group of genes with induced expression, which are involved in cell metabolism and cell cycle, suggesting increased investment of the transformed cell in cellular proliferation. We hypothesize that the decrease in expression of many diverse pathways, including the pro- and anti-apoptotic networks, cuts the energy requirements for cell maintenance, allowing energy to be diverted towards rapid cell proliferation. This study supports the hypothesis that the process of cancer transformation may be accompanied by a shift from apoptosis to necrosis.
Overall design We isolated mRNA from three independent cultures each of Keratinocytes and HPV16-immortalized keratinocytes : E, L and BP cells, grown under basal conditions. cDNA was prepared and hybridized to the Human Genome U133A Array (Affymetrix).
Contributor(s) Kravchenko-Balasha N, Mizrachy-Schwartz S, Klein S, Levitzki A
Citation(s) 19221178, 25405334
Submission date Mar 09, 2009
Last update date Jun 17, 2019
Contact name Alexander Levitzki
Phone 972-2-6585404
Fax 972-2-6512958
Organization name The Hebrew University of Jerusalem
Department Department of Biological Chemistry
Lab Prof.Levitzki
Street address Givat Ram
City Jerusalem
ZIP/Postal code 91904
Country Israel
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM378568 Keratinocytes_K_rep1
GSM378569 Keratinocytes_K_rep2
GSM378570 Keratinocytes_K_rep3
BioProject PRJNA114925

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Supplementary file Size Download File type/resource
GSE15156_RAW.tar 24.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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