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| Status |
Public on Feb 08, 2021 |
| Title |
Induction of Muscle Regenerative Multipotent Stem Cells from Human Adipocytes by PDGF-AB and 5-Azacytidine [RRBS] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Terminally differentiated murine osteocytes and adipocytes can be reprogrammed using platelet-derived growth factor–AB and 5-Azacytidine into multipotent stem cells with stromal cell characteristics. To generate a product that is amenable for therapeutic application, we have modified and optimised culture conditions to reprogram human adipocytes into induced multipotent stem cells (iMS) and expand them in vitro. The basal transcriptomes of adipocyte-derived iMS cells and matched adipose-tissue-derived mesenchymal stem cells were remarkably similar. However, there were distinct changes in histone modifications and CpG methylation at cis-regulatory regions consistent with an epigenetic landscape that was primed for tissue development and differentiation. In a non-specific tissue injury xenograft model, iMS cells contributed directly to new muscle, bone, cartilage and blood vessels with no evidence of teratogenic potential. In a cardiotoxin muscle injury model, iMS cells contributed specifically to satellite cells and myofibres without ectopic tissue formation. Taken together, human adipocyte derived iMS cells regenerate tissues in a context dependent manner without ectopic or neoplastic growth.
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| Overall design |
Distribution of regions with CpG methylation in AdMSCs and iMS cells.
This submission represents the Reduced Representation Bisulphite Sequencing (RRBS) component of the study.
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| |
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| Contributor(s) |
Yeola A, Subramanian S, Wong J, Yan FA, Chandrakanthan V, Pimanda JE |
| Citation(s) |
33523875 |
| |
| Submission date |
May 31, 2020 |
| Last update date |
Feb 08, 2021 |
| Contact name |
John E Pimanda |
| E-mail(s) |
jpimanda@unsw.edu.au
|
| Organization name |
UNSW
|
| Street address |
Cnr High and Botany St
|
| City |
Kensington |
| State/province |
NSW |
| ZIP/Postal code |
2033 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE151527 |
Induction of Muscle Regenerative Multipotent Stem Cells from Human Adipocytes by PDGF-AB and 5-Azacytidine |
|
| Relations |
| BioProject |
PRJNA636206 |
| SRA |
SRP265407 |
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