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Series GSE151402 Query DataSets for GSE151402
Status Public on Aug 11, 2020
Title Effect of Maternal Separation (MatSep) on renal vasculature isolated from male neonates (Postnatal day 10) and adult (Postnatal day 180)
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Optimal Ang II levels during postnatal life are required for a normal maturation of the rat kidney. This study investigated both short- and long-term effect of MatSep on: 1) the status of intrarenal renin-angiotensin system components, 2) the isolated renal vasculature genome-wide gene expression trajectory from neonatal to adult life, and 3) the renal vascular architecture and function.
In this dataset, gene expression changes by MatSep at P10 in renal vasculature isolated from neonates, modulate extracellular structure organization, vasculature development, inflammation and angiogenesis. The long -term effects of MatSep on renal vasculature gene expression is assessed at P180, which reveal clusters of genes with short, long, and downstream changes in expression.
 
Overall design 20 total samples were analyzed in 4 experimental groups: un-stressed (control littermates) and stressed (MatSep) neonates (P10) and adult (P180) rats (n=5 per group). We performed pair-wise comparisons and defined Early-life genes as those with expression levels at P10 > at P180 in un-stressed controls and Adult-life genes as those with expression levels at P180 > at P10. Early life MatSep affected genes were identified by pair-comparison between P10 MatSep group and P10 controls. The long-term MatSep effects on expression of genes were identified by pair-comparison between P180 MatSep group and P180 controls.
 
Contributor(s) Loria AS, Dalmasso C, Chen KC
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Submission date May 28, 2020
Last update date Aug 13, 2020
Contact name Analia S Loria
E-mail(s) analia.loria@uky.edu
Phone 8592181414
Organization name U of Kentucky
Department Pharmacology
Street address 900 S Limestone
City Lexinton
State/province KY
ZIP/Postal code 40536
Country USA
 
Platforms (1)
GPL17117 [RaGene-2_0-st] Affymetrix Rat Gene 2.0 ST Array [transcript (gene) version]
Samples (20)
GSM4579523 kidney vessels isolated at P180_no MatSep control, bio rep1
GSM4579524 kidney vessels isolated at P180_no MatSep control, bio rep2
GSM4579525 kidney vessels isolated at P180_no MatSep control, bio rep3
Relations
BioProject PRJNA635954

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151402_RAW.tar 189.3 Mb (http)(custom) TAR (of CEL)
GSE151402_processed_data.xlsx 7.6 Mb (ftp)(http) XLSX
Processed data included within Sample table
Processed data are available on Series record
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