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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 08, 2021 |
| Title |
Tcf21+ mesenchymal cells contribute to testis somatic cell development, homeostasis, and regeneration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Testicular development and function relies on interactions between somatic cells and the germline, but similar to other organs, regenerative capacity decline in aging and disease. Whether the adult testis maintains a reserve progenitor population with repair or regenerative capacity remains uncertain. Here, we characterized a recently identified mouse testis interstitial population expressing the transcription factor Tcf21. We found that Tcf21+ cells are bipotential somatic progenitors present in fetal testis and ovary, maintain adult testis homeostasis during aging, and act as reserve somatic progenitors following injury. In vitro, Tcf21+ cells are multipotent mesenchymal progenitors which form multiple somatic lineages including Leydig and myoid cells. Additionally, Tcf21+ cells resemble resident fibroblast populations reported in other organs having roles in tissue homeostasis, fibrosis, and regeneration. Our findings reveal that the testis, like other organs, maintains multipotent mesenchymal progenitors that can be leveraged in development of future therapies for hypoandrogenism and/or infertility.
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| Overall design |
Drop-seq of in-vitro differentiated Leydig cells
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| Contributor(s) |
Hammoud SS |
| Citation(s) |
34162856 |
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| Submission date |
May 28, 2020 |
| Last update date |
Aug 07, 2021 |
| Contact name |
Qianyi Ma |
| E-mail(s) |
qzm100@gmail.com
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| Organization name |
University of Michigan
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| Department |
Human Genetics
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| Street address |
1141 E Catherine St
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA635561 |
| SRA |
SRP265053 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE151337_MergedINT4LM.txt.gz |
12.2 Mb |
(ftp)(http) |
TXT |
| GSE151337_RAW.tar |
13.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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