|
| Status |
Public on Jun 15, 2020 |
| Title |
BRD4 Prevents the Accumulation of R-loops and Protects Against Transcription-Replication Collision Events and DNA Damage |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Brd4 is essential in enhancing productive polII elongation along gene bodies. Inhibition of Brd4 leads to transcription-replciation conflicts and DNA damage.
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| |
|
| Overall design |
ChIP-seq were performed for anti-Brd4, anti-gH2AX, anti-RNApolIIser2, and anti-IgG from HeLa cells treated with DMSO or JQ-1 for 16 hours.
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| |
|
| Contributor(s) |
Lam FC, Yaffe MB |
| Citation(s) |
32796829 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 ES015339 |
Protein Kinase Signaling and Cell Cycle Control |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
MICHAEL B YAFFE |
| R35 ES028374 |
Protein Kinase Signaling in the Genotoxic Stress Response |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
MICHAEL B YAFFE |
| R01 CA226898 |
RNA-Binding Proteins as Molecular Integrators that Control the Response of HGSOC to Ant-Cancer Therapies |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
MICHAEL B YAFFE |
|
| |
| Submission date |
May 22, 2020 |
| Last update date |
Aug 31, 2020 |
| Contact name |
Michael B Yaffe |
| E-mail(s) |
myaffe@mit.edu
|
| Phone |
617-452-2103
|
| Organization name |
MIT
|
| Department |
Koch Institute
|
| Lab |
76-353
|
| Street address |
500 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA634473 |
| SRA |
SRP262876 |
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