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| Status |
Public on Jul 30, 2020 |
| Title |
RNA-seq in mouse CD8+ T cells cultured with medium with or without methionine |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Abnormal epigenetic patterns correlate with effector T cell malfunction in tumors. However, their causal link is unknown. Here, we show that tumor cells disrupt methionine metabolism in CD8+ T cells, thereby lowering intracellular methionine levels and the methyl donor S-adenosylmethionine (SAM), resulting in loss of H3K79me2. Consequently, loss of H3K79me2 impaired T cell immunity. Our work reveals a novel mechanistic connection between methionine metabolism, histone patterns, and T cell immunity in the tumor microenvironment.
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| Overall design |
CD8+ T cells were cultured with fresh medium without methionine (fresh-), fresh medium with methionine (fresh+), B16F10 supernatants (Sup-), and B16F10 supernatants with methionine supplementation (Sup+) for 36h, quadruplicate per group.
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| |
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| Contributor(s) |
Bian Y, Li W, Zou W |
| Citation(s) |
32879489 |
| |
| Submission date |
May 19, 2020 |
| Last update date |
Sep 10, 2020 |
| Contact name |
Yingjie Bian |
| E-mail(s) |
yingjieb@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Surgery
|
| Street address |
109 Zina Pitcher Pl
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE150887 |
Cancer SLC43A2 alters T cell methionine metabolism and histone methylation |
|
| Relations |
| BioProject |
PRJNA633916 |
| SRA |
SRP262370 |
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