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| Status |
Public on Apr 30, 2009 |
| Title |
Inhibitor trials in chondrocytes - MAS 5.0 normalization |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Objectives: To identify similarities and differences in gene expression data in the MEK/ERK and PI3K pathways and to determine how histone modification affects these same pathways. Goal: To identify and functionally characterize novel targets of these signaling pathways in the context of chondrocyte differentiation.
Keywords: Treatment, signaling, one-colour array, signaling pathway comparison
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| Overall design |
Cartilage derived from the hind limbs of CD1 mice staged at 15.5 days of embryonic development were dissected and plated in primary chondrocyte monolayer cultures. Cells were treated with MEK/ERK pathway inhibitors (SB202190 and UO126), a PI3K inhibitor (LY294002), a histone deacetylase inhibitor (Trichostatin A) and the vehicle control (DMSO) for 24 hours. Total RNA was isolated from these samples and hybridized to Affymetrix MOE 430 2.0 chips at the London Regional Genomics facility.
A total of 15 genechips were analyzed between 4 treatments and the vehicle (DMSO) control. Three biological replicates per treatment were executed.
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| Contributor(s) |
James CG, Ulici V, Beier F, Stanton L |
| Citation(s) |
20111593 |
| |
| Submission date |
Mar 02, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
veronica ulici |
| E-mail(s) |
verau79@yahoo.com
|
| Phone |
1-519-661-3387
|
| URL |
http://www.schulich.uwo.ca/research/skeletal-web/beier/old_news.htm
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| Organization name |
University of Western Ontario
|
| Department |
Physiology and Pharmacology
|
| Lab |
Dr. Frank Beier
|
| Street address |
1151 Richmond Street
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A5C1 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA114847 |
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