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Series GSE150631 Query DataSets for GSE150631
Status Public on May 16, 2020
Title ARS2/MAGL signaling in glioblastoma stem cells promotes self-renewal and M2-like polarization of tumor-associated macrophages II
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The interplay between glioblastoma stem cells (GSCs) and tumor-associated macrophages (TAMs) promotes progression of glioblastoma multiforme (GBM). However, the detailed molecular mechanisms underlying the relationship between these two cell types remain unclear. Here, we demonstrate that ARS2 (arsenite-resistance protein 2), a zinc finger protein that is essential for early mammalian development, plays critical roles in GSC maintenance and M2-like TAM polarization. ARS2 directly activates its novel transcriptional target MGLL, encoding monoacylglycerol lipase (MAGL), to regulate the self-renewal and tumorigenicity of GSCs through production of prostaglandin E2 (PGE2), which stimulates β-catenin activation of GSC and M2-like TAM polarization. Specific knockdown of ARS2 or MAGL significantly suppressed self-renewal and tumor growth, and increased survival in a mouse intracranial xenograft model of GSCs. We identify M2-like signature downregulated by which MAGL-specific inhibitor, JZL184, increased survival rate significantly in the mouse xenograft model by blocking PGE2 production. The M2-like signature is enriched in mesenchymal subtype and predicted poor survival in GBM patients. Taken together, our results suggest that blocking the interplay between GSCs and TAMs by targeting ARS2/MAGL signaling offers a potentially novel therapeutic option for GBM patients.
Methods: X01 subcutatneous model treated vehicle or JZL184
Results: abstract copy and paste
Conclusion: abstract copy and paste
 
Overall design JZL184
 
Contributor(s) Park JB, Yin J, Kim SS, Oh YT
Citation(s) 32532977
Submission date May 15, 2020
Last update date Jun 22, 2020
Contact name Young Taek Oh
E-mail(s) yt0625@gmail.com
Organization name Samsung Medical Center
Street address 50 Irwon-dong, Gangnam-gu
City Seoul
ZIP/Postal code 135-710
Country South Korea
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (2)
GSM4555141 Vehicle of X01
GSM4555142 JZL184 of X01
Relations
BioProject PRJNA633042
SRA SRP261792

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150631_JZL184_processed_data.txt.gz 758.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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