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| Status |
Public on Apr 06, 2021 |
| Title |
HDAC4 controls senescence and aging by safeguarding the epigenetic identity and ensuring the genomic integrity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Purpose: Define HDAC4 signature
Outcome: HDAC4 is required for the repression of a senescence signature. HDAC4 KO promotes the expression of inflammatory genes, the derepression of ERVs and the accumulation of DNA damage. All together these signalling pathways lead to permanent cell-cycle arrest in low grade tumor cells and pre-transformation models, while they weaken the replicative potential of primary normal cells.
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| Overall design |
6 clones of SK-LMS-1 cells were created. Two controls: one expressing Cas9 as control, the other expressing Cas9 + sgRNA1. 4 HDAC4 KO clones were generated: 76 and 1231 (achieved through sgRNA1); 205 and 1254 (achieved through sgRNA2). Two biological replicates per clone were analyzed.
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| Contributor(s) |
Di Giorgio E, Paluvai H, Dalla E, Renzini A, Moresi V, Ranzino L, Cutano V, Picco R, Brancolini C |
| Citation(s) |
33966634, 38874468 |
| |
| Submission date |
May 12, 2020 |
| Last update date |
Sep 12, 2024 |
| Contact name |
raffaella picco |
| E-mail(s) |
raf.picco@uniud.it
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| Organization name |
university of Udine
|
| Department |
department of medical and biological sciences
|
| Lab |
bioinformatics
|
| Street address |
piazzale kolbe 4
|
| City |
udine |
| ZIP/Postal code |
33100 |
| Country |
Italy |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA632024 |
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