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Status |
Public on Oct 09, 2020 |
Title |
Gene expression for circFAM120A knockdown by RfxCas13d/BSJ-gRNA or shRNA in human 293FT cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Deep sequencing datasets are used for gene expression analysis. Knockdown of circFAM120A by BSJ-gRNA or shRNA revealed that the BSJ-gRNA-mediated knockdown showed a much higher correlation with gene expression compared to shRNA.
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Overall design |
Poly(A)+ RNA-seq of 293FT circFAM120A knockdown cells by BSJ-gRNA or shRNA are used for gene expression analysis.
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Contributor(s) |
Yang L, Chen L, Li S, Li X, Xue W |
Citation missing |
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Submission date |
May 01, 2020 |
Last update date |
Oct 11, 2020 |
Contact name |
Li Yang |
E-mail(s) |
liyang_fudan@fudan.edu.cn
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Organization name |
Fudan University
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Department |
Institutes of Biological Sciences
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Street address |
131 Dong-An Road
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City |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (8)
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GSM4509031 |
293FT cells, RfxCas13d NT replicate1 |
GSM4509032 |
293FT cells, RfxCas13d NT replicate2 |
GSM4509033 |
293FT cells, RfxCas13d circFAM120A gRNA1 replicate1 |
GSM4509034 |
293FT cells, RfxCas13d circFAM120A gRNA1 replicate2 |
GSM4509035 |
293FT cells, shRNA NT replicate1 |
GSM4509036 |
293FT cells, shRNA NT replicate2 |
GSM4509037 |
293FT cells, circFAM120A shRNA1 replicate1 |
GSM4509038 |
293FT cells, circFAM120A shRNA1 replicate2 |
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Relations |
BioProject |
PRJNA629753 |
SRA |
SRP259913 |