|
| Status |
Public on Oct 09, 2020 |
| Title |
Gene expression for circFAM120A knockdown by RfxCas13d/BSJ-gRNA or shRNA in human 293FT cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Deep sequencing datasets are used for gene expression analysis. Knockdown of circFAM120A by BSJ-gRNA or shRNA revealed that the BSJ-gRNA-mediated knockdown showed a much higher correlation with gene expression compared to shRNA.
|
| |
|
| Overall design |
Poly(A)+ RNA-seq of 293FT circFAM120A knockdown cells by BSJ-gRNA or shRNA are used for gene expression analysis.
|
| |
|
| Contributor(s) |
Yang L, Chen L, Li S, Li X, Xue W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 01, 2020 |
| Last update date |
Oct 11, 2020 |
| Contact name |
Li Yang |
| E-mail(s) |
liyang_fudan@fudan.edu.cn
|
| Organization name |
Fudan University
|
| Department |
Institutes of Biological Sciences
|
| Street address |
131 Dong-An Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (8)
|
| GSM4509031 |
293FT cells, RfxCas13d NT replicate1 |
| GSM4509032 |
293FT cells, RfxCas13d NT replicate2 |
| GSM4509033 |
293FT cells, RfxCas13d circFAM120A gRNA1 replicate1 |
| GSM4509034 |
293FT cells, RfxCas13d circFAM120A gRNA1 replicate2 |
| GSM4509035 |
293FT cells, shRNA NT replicate1 |
| GSM4509036 |
293FT cells, shRNA NT replicate2 |
| GSM4509037 |
293FT cells, circFAM120A shRNA1 replicate1 |
| GSM4509038 |
293FT cells, circFAM120A shRNA1 replicate2 |
|
| Relations |
| BioProject |
PRJNA629753 |
| SRA |
SRP259913 |
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