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| Status |
Public on Aug 14, 2020 |
| Title |
Transcriptome landscape of DNA-damage response at single-cell resolution |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The goals of this study are to investigate how the transcriptome of colon cancer cells responds to genotoxic 5FU treatments utilizing single cell RNA-seq (scRNA-seq) technology.
Methods: The collection of the single cell transcriptomes obtained from three colon cancer cell lines, RKO (CRL-2577), HCT116 (CCL-247) and SW480 (CCL-228), was generated by Drop-seq method followed by sequencing through Illumina HiSeq-4000 High-Output system.
Results: A total of 10 datasets was generated for further analysis.
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| Overall design |
A total of 10 Drop-seq experiments were run. Each experiment included three cell types (RKO, HCT116, SW480) with different 5-fluorouracil treatment which were equally mixed. Cells without drug treatment were included as controls. Controls were repeated four times and 10/50/200 μM 5FU treatment sets were repeated two times.
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| Contributor(s) |
Park SR, Kang HM, Lee JH |
| Citation(s) |
32846134 |
| |
| Submission date |
Apr 23, 2020 |
| Last update date |
Nov 27, 2020 |
| Contact name |
Jun Hee Lee |
| E-mail(s) |
leeju@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
109 Zina Pitcher Pl
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-2200 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA627695 |
| SRA |
SRP258132 |
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