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| Status |
Public on Nov 24, 2020 |
| Title |
iCLIP analysis of RNA substrates of the archaeal exosome (RNA-Seq) |
| Organism |
Saccharolobus solfataricus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, an exoribonuclease was analyzed by iCLIP. The data documents the role of the archaeal exosome as an exoribonuclease and RNA-tailing enzyme interacting with all RNA classes. Mapping of most reads to mRNAs underlines the role of exosome in mRNA turnover, which is important for adaptation of prokaryotic cells to changing environmental conditions. The clustering of crosslink sites near 5’-ends of genes suggests simultaneous binding of both RNA ends by the S. solfataricus exosome. This may serve to prevent translation of mRNAs designated to degradation in 3’-5’ direction.
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| |
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| Overall design |
1 samples, 3 replicates
|
| |
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| Contributor(s) |
Bathke J, Evguenieva-Hackenberg E |
| Citation(s) |
33198623 |
| |
| Submission date |
Apr 22, 2020 |
| Last update date |
Nov 24, 2020 |
| Contact name |
Gabriele Klug |
| E-mail(s) |
Gabriele.Klug@mikro.bio.uni-giessen.de
|
| Organization name |
Justus-Liebig University Gießen
|
| Department |
Molecular- and Microbiology
|
| Street address |
Heinrich-Buff-Ring 26-32
|
| City |
Gießen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL27060 |
Illumina NextSeq 500 (Saccharolobus solfataricus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE149143 |
iCLIP analysis of RNA substrates of the archaeal exosome |
|
| Relations |
| BioProject |
PRJNA627448 |
| SRA |
SRP257931 |
