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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 01, 2009 |
| Title |
Conjugated linoleic acid activates AMPK and reduces adiposity more effectively when used with metformin |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Trans-10, cis-12 conjugated linoleic acid (t10c12 CLA) causes dramatic reductions in white adipose tissue in mice but has had limited effectiveness in humans. Determination of the signaling pathways involved may lead to better regulation of adiposity. T10c12 CLA was found to activate AMP-activating protein kinase (AMPK), a central regulator of cell metabolism. Compound C, a potent inhibitor of AMPK, prevents many of the typical responses to treatments with t10c12 CLA including the integrated stress response (ISR), the inflammatory response, the reduction in key lipogenic transcription factors, and delipidation. Treatment of adipocytes or mice with t10c12 CLA in conjunction with AMPK activator metformin results in more delipidation than treatment with the individual chemicals. Additionally, the combination showed a reduced inflammatory response relative to a t10c12 CLA treatment alone. The combination of t10c12 CLA and metformin, widely used to treat insulin resistance and Type II diabetes, has potential as a treatment for reducing adiposity in humans.
Keywords: control/treatment
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| Overall design |
Mouse 3T3-L1 RNA for was isolated from control linoleic acid (LA) and treatment (CLA, CLA+metformin, metformin) samples for analysis on microarrays with three biological reps.
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| Contributor(s) |
Jiang S, Wang Z, Riethoven JM, Xia Y, Miner J, Fromm ME |
| Citation(s) |
19828681 |
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| Submission date |
Feb 18, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Jean-Jack M. Riethoven |
| E-mail(s) |
jeanjack@unl.edu
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| Organization name |
University of Nebraska-Lincoln
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| Department |
Beadle Center for Biotechnology
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| Lab |
Bioinformatics Core Research Facility
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| Street address |
1901 Vine Street
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| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68588-0665 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM372030 |
3T3-L1 adipocyte tissue culture, LA fed, biological rep1 |
| GSM372031 |
3T3-L1 adipocyte tissue culture, LA fed, biological rep2 |
| GSM372032 |
3T3-L1 adipocyte tissue culture, LA fed, biological rep3 |
| GSM372033 |
3T3-L1 adipocyte tissue culture, CLA fed, biological rep1 |
| GSM372034 |
3T3-L1 adipocyte tissue culture, CLA fed, biological rep2 |
| GSM372035 |
3T3-L1 adipocyte tissue culture, CLA fed, biological rep3 |
| GSM372036 |
3T3-L1 adipocyte tissue culture, metformin fed, biological rep1 |
| GSM372037 |
3T3-L1 adipocyte tissue culture, metformin fed, biological rep2 |
| GSM372038 |
3T3-L1 adipocyte tissue culture, metformin fed, biological rep3 |
| GSM372039 |
3T3-L1 adipocyte tissue culture, CLA+metformin fed, biological rep1 |
| GSM372040 |
3T3-L1 adipocyte tissue culture, CLA+metformin fed, biological rep2 |
| GSM372041 |
3T3-L1 adipocyte tissue culture, CLA+metformin fed, biological rep3 |
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| Relations |
| BioProject |
PRJNA111955 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14888_RAW.tar |
44.2 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE14888_limma_CLAMvsLA.txt |
3.1 Mb |
(ftp)(http) |
TXT |
| GSE14888_limma_CLAvsLA.txt |
3.1 Mb |
(ftp)(http) |
TXT |
| GSE14888_limma_LAMvsLA.txt |
3.1 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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