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| Status |
Public on Nov 16, 2020 |
| Title |
Peripheral blood non-canonical small non-coding RNAs as novel biomarkers in lung cancer and pulmonary tuberculosis (validation cohort) |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
One unmet challenge in current lung cancer diagnosis is to accurately differentiate lung cancer patients from those with other lung diseases with similar clinical symptoms and radiological features. Previous studies have reported cases of misdiagnosis for patients with lung cancer mimicking pulmonary tuberculosis (TB) or for TB patients with multiple lung nodules mimicking lung cancer progression, which is concerning for clinical practice in TB-endemic countries/regions. Here, we develop a molecular signature composed of non-canonical small non-coding RNAs in human peripheral blood mononuclear cells (PBMCs), including tRNA-derived small RNAs (tsRNAs), rRNA-derived small RNAs (rsRNAs), and YRNA-derived small RNAs (ysRNAs). This signature consists of i) the tsRNAs derived from tRNA-Ala, tRNA-Asn, tRNA-Leu, tRNA-Lys, and tRNA-Tyr that are upregulated in the lung cancer patients relative to the healthy controls and patients with pulmonary TB, ii) the rsRNAs derived from rRNA-5S that are upregulated in the lung cancer patients but downregulated in TB patients relative to the controls, and iii) the ysRNAs originating from YRNA-RNY1 that are downregulated in the lung cancer patients but upregulated in the TB patients compared with the controls. This diagnostic signature discriminates between healthy controls, lung cancer patients, and pulmonary TB subjects with high accuracy in both the discovery and validation cohorts. We conclude that the PBMC tsRNAs, rsRNAs, and ysRNAs are informative for both screening for and discriminating between lung cancer and pulmonary TB.
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| Overall design |
We performed small non-coding RNA sequencing for the PBMC samples collected from 35 human subjects in the validation cohort, including 12 healthy controls, 15 lung cancer patients, and 8 pulmonary TB patients.
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| Citation(s) |
33176804 |
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| Submission date |
Apr 17, 2020 |
| Last update date |
Nov 16, 2020 |
| Contact name |
Musheng Li |
| E-mail(s) |
mushengl@unr.edu
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| Phone |
7753577859
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| Organization name |
University of Nevada, Reno
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| Street address |
North Virginia Street
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| City |
Reno |
| ZIP/Postal code |
89557 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA625982 |
| SRA |
SRP256945 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE148862_count_validation_processed.txt.gz |
464.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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