|
| Status |
Public on Sep 16, 2020 |
| Title |
Sox17 and β-catenin co-occupy Wnt-responsive enhancers to govern the endodermal gene regulatory network |
| Organism |
Xenopus tropicalis |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the ChIP-seq analysis of Sox17 genome-wide binding and its loss-of-function transcriptomic analysis of both Sox17 and Ctnnb1 (beta-catenin) by RNA-seq.
|
| |
|
| Overall design |
ChIP-seq was performed to detect endogenous Sox17 binding (N&F stage 10.5) and RNA-seq was used to assess gene expression changes in Sox17 (N&F stages 9, 9.5, 10, 10.5, 11, 12) or Ctnnb1 (N&F stages 7, 8, 9, 9.5, 10, 10.5, 11, 12) in morpholino antisense oligo injected versus uninjected control embryos
|
| |
|
| Contributor(s) |
Paraiso KD, Zorn AM, Mukherjee S |
| Citation(s) |
32894225 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HD073179 |
Deciphering the gene regulatory network controlling vertebrate endodermal fates |
The Regents of the University of California |
Ken W.Y. Cho |
| P30 DK078392 |
GENE AND PROTEIN EXPRESSION CORE |
CHILDREN'S HOSPITAL MEDICAL CENTER |
S. Steven Potter |
|
| |
| Submission date |
Apr 15, 2020 |
| Last update date |
Sep 16, 2020 |
| Contact name |
Kitt D. Paraiso |
| E-mail(s) |
kparaiso@uci.edu
|
| Phone |
949-824-7950
|
| Organization name |
University of California, Irvine
|
| Department |
Department of Developmental and Cell Biology
|
| Lab |
Ken W.Y. Cho
|
| Street address |
4410 Nat Sci II, University of California, Irvine
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21875 |
Illumina HiSeq 2500 (Xenopus tropicalis) |
|
| Samples (54)
|
|
| Relations |
| BioProject |
PRJNA625510 |
| SRA |
SRP256469 |
Less...
More...