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| Status |
Public on Sep 29, 2020 |
| Title |
RNA-seq analysis of WT, CD5 KO, CD6 KO, CD5/CD6 double KO (I) and LAT KO naïve CD4+ T cells (II) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transmembrane adaptors on T cells play crucial roles in TCR signal transduction. Although the TCR-LAT signal-transduction axis occupies a central place in T cell activation, it does not work in isolation and is tuned by other T-cell surface receptors among which stand the CD5 and CD6 receptors. Here we assessed the extent of transcriptional changes that initiated by TCR-CD28 pathways in the absence of CD5, of CD6, of both CD5 and CD6, or of LAT by RNA-seq analysis.
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| Overall design |
RNA-seq analysis of untreated and stimulated naïve CD4+ T cells of wild-type, CD5 KO, CD6 KO, CD5/CD6 double KO and LAT KO. Purified T cells were stimulated with anti-CD3 plus anti-CD28 for 4 h and 20 h. WT (hDTR+) and Lat–/– (hDTR–) naïve CD4+ T cells were purified from pooled lymph nodes and spleens from Latfl-dtr maT-Cre mice using FACS AriaIII as described in Roncagalli et al (2014). There were 3 biological replicates for each conditions.
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| Contributor(s) |
Mori D, Aussel R, Malissen B |
| Citation(s) |
33125054 |
| |
| Submission date |
Apr 15, 2020 |
| Last update date |
Nov 16, 2020 |
| Contact name |
Rudy AUSSEL |
| E-mail(s) |
aussel@ciml.univ-mrs.fr
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| Organization name |
CIML
|
| Street address |
Parc Scientifique et Technologique de Luminy
|
| City |
Marseille |
| ZIP/Postal code |
13009 |
| Country |
France |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (54)
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| Relations |
| BioProject |
PRJNA625499 |
| SRA |
SRP256467 |
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