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Series GSE148686 Query DataSets for GSE148686
Status Public on Apr 15, 2020
Title A mechanism for microRNA arm switching regulated by uridylation [AQ-seq]
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Strand selection is a critical step in microRNA (miRNA) biogenesis. Although the dominant strand may alter depending on cellular contexts, the molecular mechanism and physiological significance of such alternative strand selection (or “arm switching”) remain elusive. Here we find miR-324 as one of the strongly regulated miRNAs by arm switching, and identify terminal uridylyl transferases TUT4 and TUT7 as the key regulators. Uridylation of pre-miR-324 by TUT4/7 re-positions DICER on the pre-miRNA and shifts the cleavage site. This alternative processing produces a duplex with a different terminus, from which the 3′ strand (3p) is selected instead of the 5′ strand (5p). In glioblastoma, the TUT4/7 and 3p levels are upregulated while the 5p level is reduced. Manipulation of the strand ratio is sufficient to impair glioblastoma cell proliferation. This study uncovers a role of uridylation as a molecular switch in alternative strand selection and implicates its therapeutic potential.
Overall design AQ-seq (bias-minimized sRNA-seq) libraries were constructed using total RNAs from fifteen mouse tissues (Mouse Total RNA Master Panel; Takara) as described in our previous study (Kim et al., 2019).
Contributor(s) Kim H, Kim J, Yu S, Lee YY, Park J, Choi RJ, Yoon SJ, Kang SG, Kim VN
Citation(s) 32442398
Submission date Apr 14, 2020
Last update date Jul 15, 2020
Contact name Haedong Kim
Phone +82-2-887-1343
Organization name Seoul National University
Department Biological Sciences
Street address 1 Gwanak-ro, Gwanak-gu
City Seoul
ZIP/Postal code 08826
Country South Korea
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (15)
GSM4476682 Brain AQ-seq
GSM4476683 11-day embryo AQ-seq
GSM4476684 15-day embryo AQ-seq
This SubSeries is part of SuperSeries:
GSE148687 A mechanism for microRNA arm switching regulated by uridylation
BioProject PRJNA625346
SRA SRP256394

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Supplementary file Size Download File type/resource
GSE148686_RAW.tar 70.0 Kb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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